Directed evolution: new parts and optimized function
نویسندگان
چکیده
منابع مشابه
Directed evolution: new parts and optimized function.
Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understanding, directed evolution is a powerful complement to 'rational' engineering a...
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chapter one is devoted to a moderate discussion on preliminaries, according to our requirements. chapter two which is based on our work in (24) is devoted introducting weighted semigroups (s, w), and studying some famous function spaces on them, especially the relations between go (s, w) and other function speces are invesigated. in fact this chapter is a complement to (32). one of the main fea...
15 صفحه اولOptimized expression and specific activity of IL-12 by directed molecular evolution.
DNA delivery of IL-12 has shown promise in reducing the toxic side effects associated with administration of recombinant human (h)IL-12 protein while maintaining the ability to inhibit tumor growth and abolish tumor metastases in animal models. We have developed a more potent version of IL-12 by using DNA shuffling and screening to improve its expression in human cells and specific activity on ...
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ژورنال
عنوان ژورنال: Current Opinion in Biotechnology
سال: 2009
ISSN: 0958-1669
DOI: 10.1016/j.copbio.2009.08.005